TB500 is the acetate salt form of Thymosin β4, a naturally occurring 43-amino-acid peptide found widely across many mammalian cell types and tissues. It plays a core regulatory role in actin sequestration and cytoskeleton reorganization, which underpins multiple cellular repair processes studied in preclinical laboratories. In research models, Thymosin β4 acetate demonstrates the capacity to promote cell migration, angiogenesis and tissue regeneration. It is frequently studied for wound repair, soft tissue recovery, tendon and ligament healing, and modulation of local inflammatory environments. It can help reduce excessive inflammatory response and support tissue remodeling after injury in animal research. Our TB500 acetate is synthesized by solid-phase peptide synthesis, purified by preparative HPLC. Each batch is tested with HPLC and mass spectrometry for identity confirmation and purity control. The lyophilized acetate powder greatly improves stability compared with the free peptide form, protecting biological activity during shipping and long-term frozen storage. After reconstitution with appropriate sterile solvent, the prepared solution is suitable for in vitro cell experiments and animal model research.