AICAR, also known as 5-Aminoimidazole-4-carboxamide riboside, is a well-studied nucleoside analogue widely used in biochemistry and molecular biology laboratory research. Once inside cells, AICAR is phosphorylated to form ZMP, an active metabolite capable of mimicking elevated AMP levels, which triggers allosteric activation of AMP-activated protein kinase (AMPK). AMPK serves as a core cellular energy sensor, regulating multiple downstream pathways including glucose uptake, lipid oxidation, mitochondrial biogenesis and protein synthesis.

This compound is commonly adopted as a pharmacological tool to explore AMPK-associated signaling networks. Researchers utilize AICAR in cell culture and animal models to investigate metabolic homeostasis, cellular stress response, and the molecular mechanism of energy balance regulation. Unlike many peptide reagents, AICAR is a small molecule nucleoside, which offers excellent cell permeability and stable chemical structure, bringing high repeatability for biochemical pathway exploration.

Our AICAR raw powder is synthesized and purified under strict manufacturing standards. Each batch undergoes comprehensive quality control procedures. HPLC analysis confirms high chemical purity, while related substance screening ensures low impurity content. The crystalline powder form maintains good stability under recommended storage conditions, minimizing degradation during transportation and long-term preservation.

When handling AICAR, moisture and strong light exposure should be avoided. After opening the package, it is suggested to divide the product into small sealed aliquots to reduce repeated exposure to ambient air. Dissolution should be prepared freshly before experiment to prevent solution degradation. Standard laboratory protective equipment must be worn during weighing and sample preparation.